rabbit anti spata6 proteintech 11849 1 ap wb Search Results


rabbit anti spata6 antibody  (Proteintech)
93
Proteintech rabbit anti spata6 antibody
( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, <t>SPATA6)</t> were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .
Rabbit Anti Spata6 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+spata6+proteintech+11849+1+ap+wb/SPATA6+Antibody/pmc11643634-209-0-5
Average 93 stars, based on 1 article reviews
rabbit anti spata6 antibody - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
biotech homemade α tubulin  (ABclonal Biotechnology)
97
ABclonal Biotechnology biotech homemade α tubulin
( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, <t>SPATA6)</t> were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .
Biotech Homemade α Tubulin, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+spata6+proteintech+11849+1+ap+wb/%CE%B1-Tubulin+Mouse+mAb/pm42477521-582-33-40
Average 97 stars, based on 1 article reviews
biotech homemade α tubulin - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier
rabbit anti sccpdh abclonal a2148 wb  (ABclonal Biotechnology)
93
ABclonal Biotechnology rabbit anti sccpdh abclonal a2148 wb
( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, <t>SPATA6)</t> were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .
Rabbit Anti Sccpdh Abclonal A2148 Wb, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+spata6+proteintech+11849+1+ap+wb/SCCPDH+Rabbit+pAb/pmc12019544__41467_2025_59209_MOESM1_ESM-89-167-169
Average 93 stars, based on 1 article reviews
rabbit anti sccpdh abclonal a2148 wb - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
mouse anti psmd2 santa cruz sc 271775 wb  (ABclonal Biotechnology)
93
ABclonal Biotechnology mouse anti psmd2 santa cruz sc 271775 wb
( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, <t>SPATA6)</t> were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .
Mouse Anti Psmd2 Santa Cruz Sc 271775 Wb, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+spata6+proteintech+11849+1+ap+wb/PSMD2+Rabbit+pAb/pmc12019544__41467_2025_59209_MOESM1_ESM-89-160-169
Average 93 stars, based on 1 article reviews
mouse anti psmd2 santa cruz sc 271775 wb - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
mouse anti septin 4  (ABclonal Biotechnology)
93
ABclonal Biotechnology mouse anti septin 4
( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, <t>SPATA6)</t> were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .
Mouse Anti Septin 4, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+spata6+proteintech+11849+1+ap+wb/SEPT4+Rabbit+pAb/pmc12019544__41467_2025_59209_MOESM1_ESM-89-67-70
Average 93 stars, based on 1 article reviews
mouse anti septin 4 - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
rabbit anti histone h3 abclonal a2348  (ABclonal Biotechnology)
97
ABclonal Biotechnology rabbit anti histone h3 abclonal a2348
( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, <t>SPATA6)</t> were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .
Rabbit Anti Histone H3 Abclonal A2348, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+spata6+proteintech+11849+1+ap+wb/Histone+H3+Rabbit+pAb/pmc12019544__41467_2025_59209_MOESM1_ESM-89-41-44
Average 97 stars, based on 1 article reviews
rabbit anti histone h3 abclonal a2348 - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier

Image Search Results


( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, SPATA6) were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .

Journal: eLife

Article Title: CCDC113 stabilizes sperm axoneme and head-tail coupling apparatus to ensure male fertility

doi: 10.7554/eLife.98016

Figure Lengend Snippet: ( A–C, F ) Head-tail coupling apparatus (HTCA)-associated proteins (SUN5, CENTLEIN, PMFBP1, SPATA6) were expressed alone or co-expressed with CCDC113 in HEK293T cells, and the interactions between CCDC113 and these HTCA-associated proteins were examined by co-immunoprecipitation. CCDC113 interacted with SUN5 and CENTLEIN, but did not interact with PMFBP1 and SPATA6. IB: immunoblotting; IP: immunoprecipitation. ( D ) SUN5 interacted with CCDC113. pECMV-FLAG- Ccdc113 and pEGFP-GFP- Sun5 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for immunoprecipitation (IP) with anti-GFP antibody and analyzed with anti-FLAG and anti-GFP antibodies. ( E ) CENTLEIN interacted with CCDC113. pCDNA -FLAG- Centlein and pEGFP-GFP- Ccdc113 were transfected into HEK293T cells. At 48 hr after transfection, the cells were collected for IP with anti-FLAG antibody and analyzed with anti-FLAG and anti-GFP antibodies. The % Input is displayed below the corresponding figures for quantification. n=3 independent experiments. Data are presented as mean ± SD; *p<0.05, ns indicates no significant difference. ( G ) Immunofluorescence of CCDC113 (red) and SUN5 (green) in mature spermatozoa. Nuclei were stained with DAPI (blue). ( H ) Immunofluorescence of CCDC113 (red) and CENTLEIN (green) in testicular step 13–14 spermatid. Nuclei were stained with DAPI (blue). ( I ) Immunofluorescence analysis for SPATA6 (green) and α/β-tubulin (red) was performed in Ccdc113 +/+ and Ccdc113 –/– spermatozoa. Nuclei were stained with DAPI (blue). ( J ) Quantification ratio of SPATA6 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed for each mouse. ( K ) Quantification ratio of CCDC113 on the detached sperm tail (n=3 independent experiments). At least 200 spermatozoa were analyzed from each mouse. Data are presented as mean ± SD; ***p<0.001, ****p<0.0001. ( L ) Immunofluorescence analysis for CCDC113 (red) was performed in wild-type (WT), Sun5 –/– , Centlein –/– , and Pmfbp1 –/– spermatozoa. Nuclei were stained with DAPI (blue). Figure 7—source data 1. Original files for western blot in . Figure 7—source data 2. Labelled files for western blot in .

Article Snippet: Rabbit anti-SPATA6 antibody (11849-1 AP, Proteintech) was used at a 1:100 dilution for immunofluorescence.

Techniques: Immunoprecipitation, Western Blot, Transfection, Immunofluorescence, Staining